Microinjection into Xenopus oocytes

Sigel, E (2010). Microinjection into Xenopus oocytes. In: Encyclopedia of Life Sciences. Chichester: John Wiley & Sons Ltd 10.1002/9780470015902.a0002658.pub2

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Microinjection of genetic information in the form of complimentary ribonucleic acid (cRNA) into the Xenopus oocyte leads to the functional expression of the encoded proteins. In this novel environment, exogenous proteins may be functionally characterised under well-defined conditions. Complimentary deoxyribonucleic acid (cDNA) coding for the protein may be mutated, the altered cDNA transcribed and polyadenylated in vitro to the corresponding cRNA and the function of the resulting altered protein is compared with that of the wild-type protein. The oocyte is a major expression system used in membrane protein structure–function studies. It is especially popular for expression of membrane transport proteins such as carriers and ion channels. In another approach, total messenger RNA (mRNA) may be prepared from a tissue of choice, microinjected into oocytes, the encoded functions characterised and the encoding genetic information isolated.

Item Type:	Book Section (Book Chapter)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
UniBE Contributor:	Sigel, Erwin
Publisher:	John Wiley & Sons Ltd
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:12
Last Modified:	05 Dec 2022 14:01
Publisher DOI:	10.1002/9780470015902.a0002658.pub2
URI:	https://boris.unibe.ch/id/eprint/2431 (FactScience: 204929)