Krop, EJ; Matsui, EC; Sharrow, SD; Stone, MJ; Gerber, P; van der Zee, JS; Chapman, MD; Aalberse, RC (2007). Recombinant major urinary proteins of the mouse in specific IgE and IgG testing. International archives of allergy and immunology, 144(4), pp. 296-304. Basel: Karger 10.1159/000106318
|
Text
000106318.pdf - Published Version Available under License Publisher holds Copyright. Download (364kB) | Preview |
Background: Recombinant allergens are preferred over natural allergen extracts in measuring antibodies. We tested the use of recombinant variants of the major mouse allergen Mus m 1 in detection of mouse-specific antibodies in sera of laboratory animal workers and children. Methods: Six recombinant major urinary proteins (MUPs) were produced and antibody-binding capacity was compared to natural Mus m 1 and to mouse urine extract. In a specific subset, cross-reactivity of MUP with Mus m 1 and between the different recombinant MUPs was determined. Results: For IgE antibodies, MUP8 showed high cross-reactivity with Mus m 1. MUP8-specific IgE was found in 55% of the mouse urine IgE-positive sera. Specific IgG and IgG4 antibodies against natural Mus m 1 correlated strongly with antibodies against recombinant MUP8 and were cross-reactive. IgG4 levels against MUP8 and mouse urine extract correlated, but detection of mouse urine-specific IgG4 in the absence of MUP-specific IgG4 was not uncommon. Cross-reactivity of IgG antibodies between MUP8 and Mus m 1 as well as between the different MUPs was high and inhibition varied between 54 and 99%. Conclusion: The mouse allergen Mus m 1 can be replaced in antibody testing by recombinant MUP8. Other MUPs, except MUP4, are interchangeable with MUP8. However, mouse urine extract showed better detection of both mouse-specific IgE and IgG4 levels. Other components in the mouse urine, like mouse albumin and other yet unidentified components, also induce IgE and IgG(4) antibodies.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology |
UniBE Contributor: |
Gerber, Peter |
ISSN: |
1018-2438 |
Publisher: |
Karger |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:57 |
Last Modified: |
24 Aug 2023 12:01 |
Publisher DOI: |
10.1159/000106318 |
PubMed ID: |
17652940 |
Web of Science ID: |
000250313300004 |
BORIS DOI: |
10.48350/24320 |
URI: |
https://boris.unibe.ch/id/eprint/24320 (FactScience: 48958) |
Actions (login required)
 |
Edit item |