Self-reactivity in the dimeric intravenous immunoglobulin fraction

Schaub, Alexander; Wymann, Sandra; Heller, Manfred; Ghielmetti, Marco; Beleznay, Zsuzsanne; Stadler, Beda M; Bolli, Reinhard; Miescher, Sylvia (2007). Self-reactivity in the dimeric intravenous immunoglobulin fraction. Annals of the New York Academy of Sciences, 1110, pp. 681-693. Boston, Mass.: Blackwell 10.1196/annals.1423.071

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Therapeutic intravenous immunoglobulin (IVIg) preparations contain antibodies reflecting the cumulative antigen experience of the donor population. IVIg contains variable amounts of monomeric and dimeric IgG, but there is little information available on their comparative antibody specificities. We have isolated highly purified fractions of monomeric and dimeric IgG by size-exclusion chromatography. Following treatment of all fractions at pH4, analyses by immunodot and immunocytology on human cell lines showed a preferential recognition of autoantigens in the dimeric IgG fraction. Investigation of the HEp-2 cytoplasmic proteome by 2D-PAGE, Western blot, and subsequent identification of IVIg reactive spots by mass spectrometry (LC-MS/MS) showed that IVIg recognized only a restricted set of the total proteins. Similar experiments showed that more antigens were recognized by the dimeric IgG fraction, especially when the dissociated dimer fraction was used, as compared to its monomeric counterpart. These observations are consistent with idiotype-anti-idiotype masking of auto-specific Abs in the dimeric fraction of IVIg.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology (discontinued)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Thromboselabor Kinderklinik (discontinued)

UniBE Contributor:

Wymann, Sandra; Heller, Manfred; Beleznay, Zsuzsanna; Stadler, Beda Martin and Miescher-Granger, Sylvia M.

ISSN:

0077-8923

ISBN:

17911483

Publisher:

Blackwell

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:57

Last Modified:

08 Jun 2016 10:43

Publisher DOI:

10.1196/annals.1423.071

PubMed ID:

17911483

Web of Science ID:

000250428100071

URI:

https://boris.unibe.ch/id/eprint/24639 (FactScience: 52178)

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