Vogel, Monique; Keller-Gautschi, Elsbeth; Baumann, Michael J; Amstutz, Patrick; Ruf, Christine; Kricek, Franz; Stadler, Beda M (2007). Designed ankyrin repeat proteins as anti-idiotypic-binding molecules. Annals of the New York Academy of Sciences, 1109, pp. 9-18. Boston, Mass.: Blackwell 10.1196/annals.1398.002
Full text not available from this repository.According to the network theory antibodies may act as antigens thus generating anti-idiotypic antibodies that can function as regulators of immune responses. Designed ankyrin repeat proteins (DARPins) are a new class of binding proteins and may serve as an alternative to antibodies. Selections from large DARPin libraries against the variable regions of a murine monoclonal anti-human IgE antibody, termed BSW17, yielded two highly specific anti-idiotypic DARPins both with high affinity. Their binding characteristics were comparable with these of a previously selected anti-idiotypic antibody. In vitro cell assays showed that the anti-idiotypic DARPins were able to inhibit the binding of BSW17 to cell-bound IgE and prevented BSW17 functional activity. These experiments demonstrate the possibility to isolate anti-idiotypic DARPins recognizing idiotypic determinants analogous to antibodies. In the future these DARPins may be further analyzed for their potential as putative vaccine candidates.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology [discontinued] |
UniBE Contributor: |
Vogel, Monique, Stadler, Beda Martin |
ISSN: |
0077-8923 |
ISBN: |
17785285 |
Publisher: |
Blackwell |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:57 |
Last Modified: |
05 Dec 2022 14:17 |
Publisher DOI: |
10.1196/annals.1398.002 |
PubMed ID: |
17785285 |
Web of Science ID: |
000249589100003 |
URI: |
https://boris.unibe.ch/id/eprint/24641 (FactScience: 52189) |
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