Designed ankyrin repeat proteins as anti-idiotypic-binding molecules

Vogel, Monique; Keller-Gautschi, Elsbeth; Baumann, Michael J; Amstutz, Patrick; Ruf, Christine; Kricek, Franz; Stadler, Beda M (2007). Designed ankyrin repeat proteins as anti-idiotypic-binding molecules. Annals of the New York Academy of Sciences, 1109, pp. 9-18. Boston, Mass.: Blackwell 10.1196/annals.1398.002

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According to the network theory antibodies may act as antigens thus generating anti-idiotypic antibodies that can function as regulators of immune responses. Designed ankyrin repeat proteins (DARPins) are a new class of binding proteins and may serve as an alternative to antibodies. Selections from large DARPin libraries against the variable regions of a murine monoclonal anti-human IgE antibody, termed BSW17, yielded two highly specific anti-idiotypic DARPins both with high affinity. Their binding characteristics were comparable with these of a previously selected anti-idiotypic antibody. In vitro cell assays showed that the anti-idiotypic DARPins were able to inhibit the binding of BSW17 to cell-bound IgE and prevented BSW17 functional activity. These experiments demonstrate the possibility to isolate anti-idiotypic DARPins recognizing idiotypic determinants analogous to antibodies. In the future these DARPins may be further analyzed for their potential as putative vaccine candidates.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology (discontinued)

UniBE Contributor:

Vogel, Monique and Stadler, Beda Martin

ISSN:

0077-8923

ISBN:

17785285

Publisher:

Blackwell

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:57

Last Modified:

06 Dec 2013 13:49

Publisher DOI:

10.1196/annals.1398.002

PubMed ID:

17785285

Web of Science ID:

000249589100003

URI:

https://boris.unibe.ch/id/eprint/24641 (FactScience: 52189)

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