Preparation of soluble extracts from adenovirus-infected cells for studies of RNA splicing

Mühlemann, Oliver; Akusjarvi, Goran (2007). Preparation of soluble extracts from adenovirus-infected cells for studies of RNA splicing. Methods in molecular medicine, 131, pp. 33-46. Totowa, N.J.: Humana Press

Full text not available from this repository. (Request a copy)

Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Mühlemann, Oliver

ISSN:

1543-1894

Publisher:

Humana Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:57

Last Modified:

17 Mar 2015 22:06

URI:

https://boris.unibe.ch/id/eprint/24683 (FactScience: 52815)

Actions (login required)

Edit item Edit item
Provide Feedback