Isolation and propagation of Trypanosoma brucei gambiense from sleeping sickness patients in south Sudan

Maina, Naomi; Oberle, Michael; Otieno, Charles; Kunz, Christina; Mäser, Pascal; Ndung'u, Joseph; Brun, Reto (2007). Isolation and propagation of Trypanosoma brucei gambiense from sleeping sickness patients in south Sudan. Transactions of the Royal Society of Tropical Medicine and Hygiene, 101(6), pp. 540-546. Amsterdam: Royal Society of Tropical Medicine and Hygiene 10.1016/j.trstmh.2006.11.008

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This study aimed at isolating Trypanosoma brucei gambiense from human African trypanosomiasis (HAT) patients from south Sudan. Fifty HAT patients identified during active screening surveys were recruited, most of whom (49/50) were in second-stage disease. Blood and cerebrospinal fluid samples collected from the patients were cryopreserved using Triladyl as the cryomedium. The samples were stored at -150 degrees C in liquid nitrogen vapour in a dry shipper. Eighteen patient stabilates could be propagated in immunosuppressed Mastomys natalensis and/or SCID mice. Parasitaemia was highest in SCID mice. Further subpassages in M. natalensis increased the virulence of the trypanosomes and all 18 isolates recovered from M. natalensis or SCID mice became infective to other immunosuppressed mouse breeds. A comparison of immunosuppressed M. natalensis and Swiss White, C57/BL and BALB/c mice demonstrated that all rodent breeds were susceptible after the second subpassage and developed a parasitaemia >10(6)/ml by Day 5 post infection. The highest parasitaemias were achieved in C57/BL and BALB/c mice. These results indicate that propagation of T. b. gambiense isolates after initial isolation in immunosuppressed M. natalensis or SCID mice can be done in a range of immunosuppressed rodents.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Mäser, Pascal

ISSN:

0035-9203

Publisher:

Royal Society of Tropical Medicine and Hygiene

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:57

Last Modified:

24 Oct 2019 23:01

Publisher DOI:

10.1016/j.trstmh.2006.11.008

Web of Science ID:

000246834400005

BORIS DOI:

10.7892/boris.24700

URI:

https://boris.unibe.ch/id/eprint/24700 (FactScience: 52839)

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