The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance

Kim, Do-Young; Bovet, Lucien; Maeshima, Masayoshi; Martinoia, Enrico; Lee, Youngsook (2007). The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance. The Plant Journal, 50(2), pp. 207-218. Oxford: Blackwell Science 10.1111/j.1365-313X.2007.03044.x

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Cadmium (Cd) and lead (Pb) are widespread pollutants that are toxic to plant growth. The expression of AtPDR8 was upregulated in cadmium- or lead-treated Arabidopsis thaliana. To test whether AtPDR8 is involved in heavy metal resistance, we examined transgenic Arabidopsis that over-expressed AtPDR8 and RNAi plants that exhibited a severely reduced AtPDR8 transcript level, as well as T-DNA insertion mutants of this ABC transporter. AtPDR8-over-expressing plants were more resistant to Cd(2+) or Pb(2+) than the wild-type and had lower Cd contents. In contrast, AtPDR8 RNAi transgenic plants and T-DNA insertion lines were more sensitive to Cd(2+) or Pb(2+) compared to wild-type plants and had higher Cd contents. The GFP-AtPDR8 protein was targeted to the plasma membrane, and GUS activity was present in most cells but strongest in the root hair and epidermal cells. Cd extrusion was higher in the AtPDR8-over-expressing plants in a flux assay using isolated protoplasts and radioactive (109)Cd, and was lower in the RNAi transgenic plants than in the wild-type. Together, these data strongly support a role for AtPDR8 as an efflux pump of Cd(2+) or Cd conjugates at the plasma membrane of Arabidopsis cells. As AtPDR8 has been suggested to be involved in the pathogen response and in the transport of chemicals that mediate pathogen resistance, this ABC protein is likely to transport a very broad range of substrates.

Item Type:	Journal Article (Original Article)
Division/Institute:	08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition [discontinued] 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
UniBE Contributor:	Bovet, Lucien, Martinoia, Enrico
Subjects:	500 Science > 580 Plants (Botany)
ISSN:	0960-7412
Publisher:	Blackwell Science
Language:	English
Submitter:	Peter Alfred von Ballmoos-Haas
Date Deposited:	04 Oct 2013 14:58
Last Modified:	05 Dec 2022 14:17
Publisher DOI:	10.1111/j.1365-313X.2007.03044.x
PubMed ID:	17355438
Web of Science ID:	000245697900003
URI:	https://boris.unibe.ch/id/eprint/24724 (FactScience: 52866)