Alterations in nitric oxide synthase isoforms in acute lower limb ischemia and reperfusion

Tsui, Janice C S; Baker, Daryll M; Shaw, Sidney G; Dashwood, Michael R (2007). Alterations in nitric oxide synthase isoforms in acute lower limb ischemia and reperfusion. Angiology, 58(5), pp. 586-92. Thousand Oaks, Calif.: Sage 10.1177/0003319707305466

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Alterations in nitric oxide synthase (NOS) are implicated in ischemia and ischemia-reperfusion injury. Changes in the 3 NOS isoforms in human skeletal muscle subjected to acute ischemia and reperfusion were studied. Muscle biopsies were taken from patients undergoing total knee replacement. Distribution of the specific NOS isoforms within muscle sections was studied using immunohistochemistry. NOS mRNA levels were measured using real-time reverse transcription-polymerase chain reaction and protein levels studied using Western blotting. NOS activity was also assessed using the citrulline assay. All 3 NOS isoforms were found in muscle sections associated with muscle fibers and microvessels. In muscle subjected to acute ischemia and reperfusion, NOS I/neuronal NOS mRNA and protein were elevated during reperfusion. NOS III/endothelial NOS was also upregulated at the protein level during reperfusion. No changes in NOS II/inducible NOS expression or NOS activity occurred. In conclusion, alterations in NOS I and III (neuronal NOS and endothelial NOS) at different levels occurred after acute ischemia and reperfusion in human skeletal muscle; however, this did not result in increased NOS activity. In the development of therapeutic agents based on manipulation of the NO pathway, targeting the appropriate NOS isoenzymes may be important.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Tiefenau Hospital [discontinued] > Forschungsgruppe Vasoaktive Peptide [discontinued]

UniBE Contributor:

Shaw, Sidney

ISSN:

0003-3197

ISBN:

18024942

Publisher:

Sage

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:58

Last Modified:

04 May 2014 23:17

Publisher DOI:

10.1177/0003319707305466

PubMed ID:

18024942

Web of Science ID:

000250393000011

URI:

https://boris.unibe.ch/id/eprint/24994 (FactScience: 54590)

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