Nitric oxide synthase in critically ischaemic muscle and alterations in isoform expression during revascularization surgery

Tsui, J C S; Baker, D M; Shaw, S G; Shi-Wen, X; Dashwood, M R (2008). Nitric oxide synthase in critically ischaemic muscle and alterations in isoform expression during revascularization surgery. British journal of surgery, 95(1), pp. 72-9. Bristol, UK: John Wright & Sons 10.1002/bjs.5910

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BACKGROUND: Dysfunction of the nitric oxide pathway is implicated in peripheral arterial disease. Nitric oxide synthase (NOS) isoforms and NOS activity were studied in muscle from patients with critical leg ischaemia (CLI). Alterations in NOS during revascularization surgery were also assessed. METHODS: Muscle biopsies were taken from patients with CLI undergoing amputation and also from patients undergoing femorodistal bypass at the start of surgery, after arterial clamping and following reperfusion. The presence of NOS within muscle sections was confirmed using reduced nicotinamide adenine dinucleotide phosphate diaphorase histochemistry. NOS isoform distribution was studied by immunohistochemistry. NOS mRNA and protein levels were measured using real-time reverse transcriptase-polymerase chain reaction and western blotting. NOS activity was assessed with the citrulline assay. RESULTS: All three NOS isoforms were found in muscle, associated with muscle fibres and microvessels. NOS I and III protein expression was increased in CLI (P = 0.041). During revascularization, further ischaemia and reperfusion led to a rise in NOS III protein levels (P = 0.008). NOS activity was unchanged. CONCLUSION: Alterations in NOS I and III occurred in muscle from patients with CLI and further changes occurred during bypass surgery.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Tiefenau Hospital [discontinued] > Forschungsgruppe Vasoaktive Peptide [discontinued]

UniBE Contributor:

Shaw, Sidney

ISSN:

0007-1323

ISBN:

17849374

Publisher:

John Wright & Sons

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:58

Last Modified:

04 May 2014 23:17

Publisher DOI:

10.1002/bjs.5910

PubMed ID:

17849374

Web of Science ID:

000253256400009

URI:

https://boris.unibe.ch/id/eprint/24995 (FactScience: 54592)

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