Lymphocyte blastogenic response to ovalbumin in a model for canine allergy

Dévaud, N.; Hall, J.A.; Gaschen, F.; Vallan, C.; Doherr, M.G.; Williamson, L.; Peel, J.E.; Burgener, I.A. (2009). Lymphocyte blastogenic response to ovalbumin in a model for canine allergy. Veterinary journal, 181(2), pp. 178-186. Amsterdam: Elsevier 10.1016/j.tvjl.2008.02.006

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Lymphocyte stimulation tests (LST) were performed in five dogs sensitised with ovalbumin (OVA) and seven healthy dogs. In addition, all five OVA-sensitised and two control dogs were tested after two in vivo provocations with OVA-containing eye drops. The isolated cells were suspended in culture media containing OVA and were cultured for up to 12 days. Proliferation was measured as reduction in 5,6-carboxylfluorescein diacetate succinimidyl ester (CFSE) intensity by flow cytometry on days 0, 3, 6, 9 and 12. A cell proliferation index (CPI) for each day and the area under the curve (AUC) of the CPI was calculated for each dog. All OVA-sensitised dogs demonstrated increased erythema after conjunctival OVA application. The presence of OVA-specific lymphocytes was demonstrated in 2/5 OVA-sensitised dogs before and 4/5 after in vivo provocation. Using the AUC, the difference between OVA-sensitised and control dogs was significant in all three LST before in vivo provocation (P<0.05) and borderline significant (P=0.053) in 2/3 LST after provocation. The most significant difference in CPI was observed after 9 days of culture (P=0.001). This pilot study indicates that the LST allows detection of rare antigen specific memory T-cells in dogs previously sensitised to, but not concurrently undergoing challenge by a specific antigen.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DVK - Clinical Research (discontinued)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research

UniBE Contributor:

Doherr, Marcus and Burgener, Iwan

ISSN:

1090-0233

Publisher:

Elsevier

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:00

Last Modified:

03 Feb 2015 09:23

Publisher DOI:

10.1016/j.tvjl.2008.02.006

PubMed ID:

18397835

Web of Science ID:

000266734200013

URI:

https://boris.unibe.ch/id/eprint/26048 (FactScience: 63365)

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