Inhibition of iodine organification and regulation of follicular size in rat thyroid tissue in vitro

Glaser, C; Marti, U; Bürgi-Saville, ME; Ruchti, C; Gebauer, M; Büchler, MW; Gerber, H; Bürgi, U; Peter, HJ (1999). Inhibition of iodine organification and regulation of follicular size in rat thyroid tissue in vitro. Endocrine, 11(2), pp. 165-70. Basingstoke: Humana Press 10.1385/ENDO:11:2:165

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The factors mediating the accumulation of thyroglobulin are of great importance to the understanding of the pathogenesis of human and experimentally induced colloid goiters. To elucidate further the underlying cellular mechanism, thyroid fragments from newborn rats were incorporated into semisolid alginate beads and were cultured as three-dimensional organoids for up to 21 d. In five parallel cultures, the medium contained either no supplements (group A), Nal (group B), thyroid-stimulating hormone (TSH) (group C), Nal plus TSH in the same concentrations as B and C (group D), or Nal and TSH (as in group D) plus methimazole (MMI, group E). The thyroid organoids maintained morphological integrity, functional activity, and ability to proliferate in vitro. Addition of iodine to the cultures significantly increased mean (+/-SEM) follicular diameters from 19.5 +/- 0.7 microm in controls to 33.9 +/- 2.2 microm (p < 0.0001) when NaI was added alone (group B), and 30.4 +/- 1.7 microm (p < 0.0001) when combined with TSH (group D). The effect of NaI on follicular size was abolished by MMI (group E, follicular diameter 23.5 +/- 1.3 microm). The results presented support the recent finding, using a rat colloid goiter model, that not only TSH but also iodine organification or its inhibition are important factors in modulating follicular morphology.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of General Internal Medicine (DAIM) > Clinic of General Internal Medicine

UniBE Contributor:

Bürgi, Ulrich

ISSN:

1355-008X

ISBN:

10709764

Publisher:

Humana Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:01

Last Modified:

25 Oct 2019 02:34

Publisher DOI:

10.1385/ENDO:11:2:165

PubMed ID:

10709764

Web of Science ID:

000084293300009

BORIS DOI:

10.7892/boris.26577

URI:

https://boris.unibe.ch/id/eprint/26577 (FactScience: 73531)

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