Brandt, Sabine; Haralambus, Rhea; Schoster, Angelika; Kirnbauer, Reinhard; Stanek, Christian (2008). Peripheral blood mononuclear cells represent a reservoir of bovine papillomavirus DNA in sarcoid-affected equines. Journal of general virology, 89(Pt 6), pp. 1390-5. Reading: Society for General Microbiology 10.1099/vir.0.83568-0
Full text not available from this repository.Bovine papillomaviruses of types 1 and 2 (BPV-1 and -2) chiefly contribute to equine sarcoid pathogenesis. However, the mode of virus transmission and the presence of latent infections are largely unknown. This study established a PCR protocol allowing detection of <or=10 copies of the BPV-1/-2 genes E5 and L1. Subsequent screening of peripheral blood mononuclear cell (PBMC) DNA derived from horses with and without BPV-1/2-induced skin lesions demonstrated the exclusive presence of E5, but not L1, in PBMCs of BPV-1/2-infected equines. To validate this result, a blind PCR was performed from enciphered PBMC DNA derived from 66 horses, revealing E5 in the PBMCs of three individuals with confirmed sarcoids, whereas the remaining 63 sarcoid-free animals were negative for this gene. L1 could not be detected in any PBMC DNA, suggesting either deletion or interruption of this gene in PBMCs of BPV-1/-2-infected equines. These results support the hypothesis that PBMCs may serve as host cells for BPV-1/-2 DNA and contribute to virus latency.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic and Policlinic for Anaesthesiology and Pain Therapy |
UniBE Contributor: |
Brandt, Sebastian |
ISSN: |
0022-1317 |
ISBN: |
18474554 |
Publisher: |
Society for General Microbiology |
Language: |
English |
Submitter: |
Jeannie Wurz |
Date Deposited: |
04 Oct 2013 15:01 |
Last Modified: |
05 Dec 2022 14:19 |
Publisher DOI: |
10.1099/vir.0.83568-0 |
PubMed ID: |
18474554 |
Web of Science ID: |
000256687200008 |
URI: |
https://boris.unibe.ch/id/eprint/26665 (FactScience: 81092) |
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