Peripheral blood mononuclear cells represent a reservoir of bovine papillomavirus DNA in sarcoid-affected equines

Brandt, Sabine; Haralambus, Rhea; Schoster, Angelika; Kirnbauer, Reinhard; Stanek, Christian (2008). Peripheral blood mononuclear cells represent a reservoir of bovine papillomavirus DNA in sarcoid-affected equines. Journal of general virology, 89(Pt 6), pp. 1390-5. Reading: Society for General Microbiology 10.1099/vir.0.83568-0

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Bovine papillomaviruses of types 1 and 2 (BPV-1 and -2) chiefly contribute to equine sarcoid pathogenesis. However, the mode of virus transmission and the presence of latent infections are largely unknown. This study established a PCR protocol allowing detection of <or=10 copies of the BPV-1/-2 genes E5 and L1. Subsequent screening of peripheral blood mononuclear cell (PBMC) DNA derived from horses with and without BPV-1/2-induced skin lesions demonstrated the exclusive presence of E5, but not L1, in PBMCs of BPV-1/2-infected equines. To validate this result, a blind PCR was performed from enciphered PBMC DNA derived from 66 horses, revealing E5 in the PBMCs of three individuals with confirmed sarcoids, whereas the remaining 63 sarcoid-free animals were negative for this gene. L1 could not be detected in any PBMC DNA, suggesting either deletion or interruption of this gene in PBMCs of BPV-1/-2-infected equines. These results support the hypothesis that PBMCs may serve as host cells for BPV-1/-2 DNA and contribute to virus latency.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic and Policlinic for Anaesthesiology and Pain Therapy

UniBE Contributor:

Brandt, Sebastian

ISSN:

0022-1317

ISBN:

18474554

Publisher:

Society for General Microbiology

Language:

English

Submitter:

Jeannie Wurz

Date Deposited:

04 Oct 2013 15:01

Last Modified:

23 Jan 2018 12:17

Publisher DOI:

10.1099/vir.0.83568-0

PubMed ID:

18474554

Web of Science ID:

000256687200008

URI:

https://boris.unibe.ch/id/eprint/26665 (FactScience: 81092)

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