Brandt, S; Haralambus, R; Shafti-Keramat, S; Steinborn, R; Stanek, C; Kirnbauer, R (2008). A subset of equine sarcoids harbours BPV-1 DNA in a complex with L1 major capsid protein. Virology, 375(2), pp. 433-41. New York, N.Y.: Academic Press 10.1016/j.virol.2008.02.014
Full text not available from this repository.Bovine papillomavirus type 1 or 2 (BPV-1, BPV-2) are accepted causal factors in equine sarcoid pathogenesis. Whereas viral genomes are consistently found and expressed within lesions, intact virions have never been detected, thus permissiveness of sarcoids for BPV-1 replication remains unclear. To reassess this issue, an immunocapture PCR (IC/PCR) was established using L1-specific antibodies to capture L1-DNA complexes followed by amplification of the viral genome. Following validation of the assay, 13 sarcoid-bearing horses were evaluated by IC/PCR. Samples were derived from 21 tumours, 4 perilesional/intact skin biopsies, and 1 serum. Tissue extracts from sarcoid-free equines served as controls. IC/PCR scored positive in 14/24 (58.3%) specimens obtained from sarcoid-patients, but negative for controls. Quantitative IC/PCR demonstrated <125 immunoprecipitable viral genomes/50 microl extract for the majority of specimens. Moreover, full-length BPV-1 genomes were detected in a complex with L1 proteins. These complexes may correspond to virion precursors or intact virions.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic and Policlinic for Anaesthesiology and Pain Therapy |
UniBE Contributor: |
Brandt, Sebastian |
ISSN: |
0042-6822 |
ISBN: |
18395238 |
Publisher: |
Academic Press |
Language: |
English |
Submitter: |
Jeannie Wurz |
Date Deposited: |
04 Oct 2013 15:01 |
Last Modified: |
05 Dec 2022 14:19 |
Publisher DOI: |
10.1016/j.virol.2008.02.014 |
PubMed ID: |
18395238 |
Web of Science ID: |
000256106200013 |
URI: |
https://boris.unibe.ch/id/eprint/26666 (FactScience: 81094) |
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