A subset of equine sarcoids harbours BPV-1 DNA in a complex with L1 major capsid protein

Brandt, S; Haralambus, R; Shafti-Keramat, S; Steinborn, R; Stanek, C; Kirnbauer, R (2008). A subset of equine sarcoids harbours BPV-1 DNA in a complex with L1 major capsid protein. Virology, 375(2), pp. 433-41. New York, N.Y.: Academic Press 10.1016/j.virol.2008.02.014

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Bovine papillomavirus type 1 or 2 (BPV-1, BPV-2) are accepted causal factors in equine sarcoid pathogenesis. Whereas viral genomes are consistently found and expressed within lesions, intact virions have never been detected, thus permissiveness of sarcoids for BPV-1 replication remains unclear. To reassess this issue, an immunocapture PCR (IC/PCR) was established using L1-specific antibodies to capture L1-DNA complexes followed by amplification of the viral genome. Following validation of the assay, 13 sarcoid-bearing horses were evaluated by IC/PCR. Samples were derived from 21 tumours, 4 perilesional/intact skin biopsies, and 1 serum. Tissue extracts from sarcoid-free equines served as controls. IC/PCR scored positive in 14/24 (58.3%) specimens obtained from sarcoid-patients, but negative for controls. Quantitative IC/PCR demonstrated <125 immunoprecipitable viral genomes/50 microl extract for the majority of specimens. Moreover, full-length BPV-1 genomes were detected in a complex with L1 proteins. These complexes may correspond to virion precursors or intact virions.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic and Policlinic for Anaesthesiology and Pain Therapy

UniBE Contributor:

Brandt, Sebastian

ISSN:

0042-6822

ISBN:

18395238

Publisher:

Academic Press

Language:

English

Submitter:

Jeannie Wurz

Date Deposited:

04 Oct 2013 15:01

Last Modified:

23 Jan 2018 12:17

Publisher DOI:

10.1016/j.virol.2008.02.014

PubMed ID:

18395238

Web of Science ID:

000256106200013

URI:

https://boris.unibe.ch/id/eprint/26666 (FactScience: 81094)

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