Similarities in the Subgingival Microbiota Assessed by a Curet Sampling Method at Sites With Chronic Periodontitis

Persson, Gösta Rutger; Weibel, Marianne; Hirschi, Regula; Katsoulis, Joannis (2008). Similarities in the Subgingival Microbiota Assessed by a Curet Sampling Method at Sites With Chronic Periodontitis. Journal of periodontology, 79(12), pp. 2290-2296. Chicago, Ill.: American Academy of Periodontology 10.1902/jop.2008.080142

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Background: The goal of this study was to determine whether site-specific differences in the subgingival microbiota could be detected by the checkerboard method in subjects with periodontitis. Methods: Subjects with at least six periodontal pockets with a probing depth (PD) between 5 and 7 mm were enrolled in the study. Subgingival plaque samples were collected with sterile curets by a single-stroke procedure at six selected periodontal sites from 161 subjects (966 subgingival sites). Subgingival bacterial samples were assayed with the checkerboard DNA-DNA hybridization method identifying 37 species. Results: Probing depths of 5, 6, and 7 mm were found at 50% (n = 483), 34% (n = 328), and 16% (n = 155) of sites, respectively. Statistical analysis failed to demonstrate differences in the sum of bacterial counts by tooth type (P = 0.18) or specific location of the sample (P = 0.78). With the exceptions of Campylobacter gracilis (P <0.001) and Actinomyces naeslundii (P <0.001), analysis by general linear model multivariate regression failed to identify subject or sample location factors as explanatory to microbiologic results. A trend of difference in bacterial load by tooth type was found for Prevotella nigrescens (P <0.01). At a cutoff level of >/=1.0 x 10(5), Porphyromonas gingivalis and Tannerella forsythia (previously T. forsythensis) were present at 48.0% to 56.3% and 46.0% to 51.2% of sampled sites, respectively. Conclusions: Given the similarities in the clinical evidence of periodontitis, the presence and levels of 37 species commonly studied in periodontitis are similar, with no differences between molar, premolar, and incisor/cuspid subgingival sites. This may facilitate microbiologic sampling strategies in subjects during periodontal therapy.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Prosthodontics (discontinued)
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Persson, Gösta Rutger; Weibel, Marianne; Hirschi, Regula and Katsoulis, Joannis

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-3492

Publisher:

American Academy of Periodontology

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 15:02

Last Modified:

25 Jan 2017 12:17

Publisher DOI:

10.1902/jop.2008.080142

PubMed ID:

19053919

Web of Science ID:

000261710900011

BORIS DOI:

10.7892/boris.26779

URI:

https://boris.unibe.ch/id/eprint/26779 (FactScience: 88004)

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