Keymel, S; Kalka, C; Rassaf, T; Yeghiazarians, Y; Kelm, M; Heiss, C (2008). Impaired endothelial progenitor cell function predicts age-dependent carotid intimal thickening. Basic research in cardiology, 103(6), pp. 582-6. Heidelberg: Springer-Medizin-Verlag 10.1007/s00395-008-0742-z
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Keymel2008_Article_ImpairedEndothelialProgenitorC.pdf - Published Version Available under License Publisher holds Copyright. Download (197kB) | Preview |
OBJECTIVES: We investigated whether qualitative or quantitative alterations of the endothelial progenitor cell (EPC) pool predict age-related structural vessel wall changes. BACKGROUND: We have previously shown that age-related endothelial dysfunction is accompanied by qualitative rather than quantitative changes of EPCs. Animal studies suggest that impaired EPC functions lead to accelerated arterial intimal thickening. METHODS: Intima-media thickness (IMT) was measured in the common carotid artery in our previously published groups of younger (25 +/- 1 years, n = 20) and older (61 +/- 2 years, n = 20) healthy non-smoking volunteers without arterial hypertension, hypercholesterolemia, and diabetes mellitus. Endothelial progenitor cells (EPCs, KDR(+)/CD34(+) and KDR(+)/CD133(+)) were counted in peripheral blood using flow cytometry. In ex vivo expanded EPCs, the function was determined as chemotaxis to VEGF, proliferation, and survival. RESULTS: We observed thicker IMT in older as compared to younger subjects (0.68 +/- 0.03 mm Vs. 0.48 +/- 0.02 mm, P < 0.001). Importantly, there were significant inverse univariate correlations between IMT, EPC chemotaxis, and survival (r = -0.466 P < 0.05; r = -0.463, P < 0.01). No correlation was observed with numbers of circulating EPCs. Multivariate regression analysis revealed that age, mean arterial pressure and migration of EPCs were independent predictors of IMT (R (2 )= 0.58). CONCLUSION: Impaired EPC function may lead to accelerated vascular remodeling due to chronic impairment of endothelial maintenance.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Angiology |
UniBE Contributor: |
Kalka, Christoph |
ISSN: |
0300-8428 |
ISBN: |
582-6 |
Publisher: |
Springer-Medizin-Verlag |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:02 |
Last Modified: |
05 Dec 2022 14:19 |
Publisher DOI: |
10.1007/s00395-008-0742-z |
Web of Science ID: |
000259935300009 |
BORIS DOI: |
10.7892/boris.26886 |
URI: |
https://boris.unibe.ch/id/eprint/26886 (FactScience: 91618) |
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