Impaired endothelial progenitor cell function predicts age-dependent carotid intimal thickening

Keymel, S; Kalka, C; Rassaf, T; Yeghiazarians, Y; Kelm, M; Heiss, C (2008). Impaired endothelial progenitor cell function predicts age-dependent carotid intimal thickening. Basic research in cardiology, 103(6), pp. 582-6. Heidelberg: Springer-Medizin-Verlag 10.1007/s00395-008-0742-z

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OBJECTIVES: We investigated whether qualitative or quantitative alterations of the endothelial progenitor cell (EPC) pool predict age-related structural vessel wall changes. BACKGROUND: We have previously shown that age-related endothelial dysfunction is accompanied by qualitative rather than quantitative changes of EPCs. Animal studies suggest that impaired EPC functions lead to accelerated arterial intimal thickening. METHODS: Intima-media thickness (IMT) was measured in the common carotid artery in our previously published groups of younger (25 +/- 1 years, n = 20) and older (61 +/- 2 years, n = 20) healthy non-smoking volunteers without arterial hypertension, hypercholesterolemia, and diabetes mellitus. Endothelial progenitor cells (EPCs, KDR(+)/CD34(+) and KDR(+)/CD133(+)) were counted in peripheral blood using flow cytometry. In ex vivo expanded EPCs, the function was determined as chemotaxis to VEGF, proliferation, and survival. RESULTS: We observed thicker IMT in older as compared to younger subjects (0.68 +/- 0.03 mm Vs. 0.48 +/- 0.02 mm, P < 0.001). Importantly, there were significant inverse univariate correlations between IMT, EPC chemotaxis, and survival (r = -0.466 P < 0.05; r = -0.463, P < 0.01). No correlation was observed with numbers of circulating EPCs. Multivariate regression analysis revealed that age, mean arterial pressure and migration of EPCs were independent predictors of IMT (R (2 )= 0.58). CONCLUSION: Impaired EPC function may lead to accelerated vascular remodeling due to chronic impairment of endothelial maintenance.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Angiology

UniBE Contributor:

Kalka, Christoph

ISSN:

0300-8428

ISBN:

582-6

Publisher:

Springer-Medizin-Verlag

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:02

Last Modified:

25 May 2020 14:38

Publisher DOI:

10.1007/s00395-008-0742-z

Web of Science ID:

000259935300009

BORIS DOI:

10.7892/boris.26886

URI:

https://boris.unibe.ch/id/eprint/26886 (FactScience: 91618)

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