Hydrogel-based engineered skeletal muscle grafts normalize heart function early after myocardial infarction

Giraud, Marie-Noëlle; Ayuni, Erick; Cook, Stéphane; Siepe, Matthias; Carrel, Thierry P; Tevaearai, Hendrik T (2008). Hydrogel-based engineered skeletal muscle grafts normalize heart function early after myocardial infarction. Artificial organs, 32(9), pp. 692-700. Malden, Mass.: Wiley-Blackwell 10.1111/j.1525-1594.2008.00595.x

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Tissue engineering represents an attractive approach for the treatment of congestive heart failure. The influence of the differentiation of myogenic graft for functional recovery is not defined. We engineered a biodegradable skeletal muscle graft (ESMG) tissue and investigated its functional effect after implantation on the epicardium of an infarcted heart segment. ESMGs were synthesized by mixing collagen (2 mg/mL), Matrigel (2 mg/mL), and rat skeletal muscle cells (10(6)). Qualitative and quantitative aspects of ESMGs were optimized. Two weeks following coronary ligation, the animals were randomized in three groups: ESMG glued to the epicardial surface with fibrin (ESMG, n = 7), fibrin alone (fibrin, n = 5), or sham operation (sham, n = 4). Echocardiography, histology, and immunostaining were performed 4 weeks later. A cohesive three-dimensional tissular structure formed in vitro within 1 week. Myoblasts differentiated into randomly oriented myotubes. Four weeks postimplantation, ESMGs were vascularized and invaded by granulation tissue. Mean fractional shortening (FS) was, however, significantly increased in the ESMG group as compared with preimplantation values (42 +/- 6 vs. 33 +/- 5%, P < 0.05) and reached the values of controlled noninfarcted animals (control, n = 5; 45 +/- 3%; not significant). Pre- and postimplantation FS did not change over these 4 weeks in the sham group and the fibrin-treated animals. This study showed that it is possible to improve systolic heart function following myocardial infarction through implantation of differentiated muscle fibers seeded on a gel-type scaffold despite a low rate of survival.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Heart Surgery 04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiology
UniBE Contributor:	Giraud, Marie-Noelle, Cook, Stéphane, Carrel, Thierry, Tevaearai, Hendrik
ISSN:	0160-564X
ISBN:	18684206
Publisher:	Wiley-Blackwell
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:02
Last Modified:	27 Feb 2024 14:29
Publisher DOI:	10.1111/j.1525-1594.2008.00595.x
PubMed ID:	18684206
Web of Science ID:	000259270100004
URI:	https://boris.unibe.ch/id/eprint/26935 (FactScience: 99152)