Characterization of three human sec14p-like proteins: alpha-tocopherol transport activity and expression pattern in tissues

Zingg, Jean-Marc; Kempna, Petra; Paris, Marcel; Reiter, Elke; Villacorta, Luis; Cipollone, Rita; Munteanu, Adelina; De Pascale, Clara; Menini, Stefano; Cueff, Alexandra; Arock, Michel; Azzi, Angelo; Ricciarelli, Roberta (2008). Characterization of three human sec14p-like proteins: alpha-tocopherol transport activity and expression pattern in tissues. Biochimie, 90(11-12), pp. 1703-1715. Paris: Elsevier Masson SAS 10.1016/j.biochi.2008.07.008

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Three closely related human sec14p-like proteins (hTAP1, 2, and 3, or SEC14L2, 3, and 4, respectively) have been described. These proteins may participate in intracellular lipid transport (phospholipids, squalene, tocopherol analogues and derivatives) or influence regulatory lipid-dependent events. Here, we show that the three recombinant hTAP proteins associate with the Golgi apparatus and mitochondria, and enhance the in vitro transport of radioactively labeled alpha-tocopherol to mitochondria in the same order of magnitude as the human alpha-tocopherol transfer protein (alpha-TTP). hTAP1 and hTAP2 are expressed in several cell lines, whereas the expression level of hTAP3 is low. Expression of hTAP1 is induced in human umbilical cord blood-derived mast cells upon differentiation by interleukin 4. In tissues, the three hTAPs are detectable ubiquitously at low level; pronounced and localized expression is found for hTAP2 and hTAP3 in the perinuclear region in cerebellum, lung, liver and adrenal gland. hTAP3 is well expressed in the epithelial duct cells of several glands, in ovary in endothelial cells of small arteries as well as in granulosa and thecal cells, and in testis in Leydig cells. Thus, the three hTAPs may mediate lipid uptake, secretion, presentation, and sub-cellular localization in a tissue-specific manner, possibly using organelle- and enzyme-specific docking sites.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine

UniBE Contributor:

Kempna, Petra






Elsevier Masson SAS




Factscience Import

Date Deposited:

04 Oct 2013 15:03

Last Modified:

14 Jul 2015 11:01

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URI: (FactScience: 105030)

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