Millward, Thomas A; Heitzmann, Markus; Bill, Kurt; Längle, Ulrich; Schumacher, Peter; Forrer, Kurt (2008). Effect of constant and variable domain glycosylation on pharmacokinetics of therapeutic antibodies in mice. Biologicals, 36(1), pp. 41-7. London: Elsevier 10.1016/j.biologicals.2007.05.003
Full text not available from this repository. (Request a copy)Previous studies on the effect of glycosylation on the elimination rate of antibodies have produced conflicting results. Here, we performed pharmacokinetic studies in mice with two preparations of a monoclonal IgG1 antibody enriched for complex type or high mannose type oligosaccharides at the Fc glycosylation site. No significant difference in the serum half-life was found between the two antibody glycoforms, nor was any difference observed in the serum half-lives of different complex type glycoforms. To evaluate the influence of glycosylation within the variable domain, a second monoclonal antibody, glycosylated in both the Fc and Fv domains, was separated into fractions containing different amounts of Fv-associated sialic acid and administered to mice. Again, no significant difference was found in the clearance rates of variants carrying different amounts of Fv-associated sialic acid or lacking Fv-glycosylation. These results suggest that glycosylation has little or no impact on the pharmacokinetic behavior of these two monoclonal antibodies in mice.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic and Policlinic for Anaesthesiology and Pain Therapy |
UniBE Contributor: |
Schumacher, Peter |
ISSN: |
1045-1056 |
ISBN: |
17890101 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Jeannie Wurz |
Date Deposited: |
04 Oct 2013 15:03 |
Last Modified: |
05 Dec 2022 14:19 |
Publisher DOI: |
10.1016/j.biologicals.2007.05.003 |
PubMed ID: |
17890101 |
Web of Science ID: |
000252987900006 |
URI: |
https://boris.unibe.ch/id/eprint/27291 (FactScience: 105917) |