Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase

Ulrich, Martina; Petre, Alina; Youhnovski, Nikolay; Prömm, Franziska; Schirle, Markus; Schumm, Michael; Pero, Ralph S; Doyle, Alfred; Checkel, James; Kita, Hirohito; Thiyagarajan, Nethaji; Acharya, K Ravi; Schmid-Grendelmeier, Peter; Simon, Hans-Uwe; Schwarz, Heinz; Tsutsui, Masato; Shimokawa, Hiroaki; Bellon, Gabriel; Lee, James J; Przybylski, Michael; ... (2008). Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase. Journal of biological chemistry, 283(42), pp. 28629-40. Bethesda, Md.: American Society for Biochemistry and Molecular Biology 10.1074/jbc.M801196200

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Nitration of tyrosine residues has been observed during various acute and chronic inflammatory diseases. However, the mechanism of tyrosine nitration and the nature of the proteins that become tyrosine nitrated during inflammation remain unclear. Here we show that eosinophils but not other cell types including neutrophils contain nitrotyrosine-positive proteins in specific granules. Furthermore, we demonstrate that the human eosinophil toxins, eosinophil peroxidase (EPO), major basic protein, eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP), and the respective murine toxins, are post-translationally modified by nitration at tyrosine residues during cell maturation. High resolution affinity-mass spectrometry identified specific single nitration sites at Tyr349 in EPO and Tyr33 in both ECP and EDN. ECP and EDN crystal structures revealed and EPO structure modeling suggested that the nitrated tyrosine residues in the toxins are surface exposed. Studies in EPO(-/-), gp91phox(-/-), and NOS(-/-) mice revealed that tyrosine nitration of these toxins is mediated by EPO in the presence of hydrogen peroxide and minute amounts of NOx. Tyrosine nitration of eosinophil granule toxins occurs during maturation of eosinophils, independent of inflammation. These results provide evidence that post-translational tyrosine nitration is unique to eosinophils.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

Simon, Hans-Uwe

ISSN:

0021-9258

ISBN:

18694936

Publisher:

American Society for Biochemistry and Molecular Biology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:03

Last Modified:

04 May 2014 23:19

Publisher DOI:

10.1074/jbc.M801196200

PubMed ID:

18694936

Web of Science ID:

000259969300065

URI:

https://boris.unibe.ch/id/eprint/27535 (FactScience: 108754)

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