A newly developed in vitro model of the human epithelial airway barrier to study the toxic potential of nanoparticles

Rothen-Rutishauser, Barbara; Mueller, Loretta; Blank, Fabian; Brandenberger, Christina; Muehlfeld, Christian; Gehr, Peter (2008). A newly developed in vitro model of the human epithelial airway barrier to study the toxic potential of nanoparticles. ALTEX - Alternativen zu Tierexperimenten, 25(3), pp. 191-6. Heidelberg: Spektrum Akademischer Verlag

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The potential health effects of inhaled engineered nanoparticles are almost unknown. To avoid and replace toxicity studies with animals, a triple cell co-culture system composed of epithelial cells, macrophages and dendritic cells was established, which simulates the most important barrier functions of the epithelial airway. Using this model, the toxic potential of titanium dioxide was assessed by measuring the production of reactive oxygen species and the release of tumour necrosis factor alpha. The intracellular localisation of titanium dioxide nanoparticles was analyzed by energy filtering transmission electron microscopy. Titanium dioxide nanoparticles were detected as single particles without membranes and in membrane-bound agglomerates. Cells incubated with titanium dioxide particles showed an elevated production of reactive oxygen species but no increase of the release of tumour necrosis factor alpha. Our in vitro model of the epithelial airway barrier offers a valuable tool to study the interaction of particles with lung cells at a nanostructural level and to investigate the toxic potential of nanoparticles.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

UniBE Contributor:

Rothen-Rutishauser, Barbara; Blank, Fabian; Brandenberger, Christina and Gehr, Peter

ISSN:

0946-7785

ISBN:

18841315

Publisher:

Spektrum Akademischer Verlag

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:03

Last Modified:

04 May 2014 23:19

PubMed ID:

18841315

Web of Science ID:

000259734300004

URI:

https://boris.unibe.ch/id/eprint/27554 (FactScience: 108817)

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