A novel cell compatible impingement system to study in vitro drug absorption from dry powder aerosol formulations

Bur, Michael; Rothen-Rutishauser, Barbara; Huwer, Hanno; Lehr, Claus-Michael (2009). A novel cell compatible impingement system to study in vitro drug absorption from dry powder aerosol formulations. European journal of pharmaceutics and biopharmaceutics, 72(2), pp. 350-7. Amsterdam: Elsevier 10.1016/j.ejpb.2008.07.019

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A modified Astra type multistage liquid impinger (MSLI) with integrated bronchial cell monolayers was used to study deposition and subsequent drug absorption on in vitro models of the human airway epithelial barrier. Inverted cell culture of Calu-3 cells on the bottom side of cell culture filter inserts was integrated into a compendial MSLI. Upside down cultivation did not impair the barrier function, morphology and viability of Calu-3 cells. Size selective deposition with subsequent absorption was studied for three different commercially available dry powder formulations of salbutamol sulphate and budesonide. After deposition without size separation the absorption rates from the aerosol formulations differed but correlated with the size of the carrier lactose particles. However, after deposition in the MSLI, simulating relevant impaction and causing the separation of small drug crystals from the carrier lactose, the absorption rates of the three formulations were identical, confirming the bioequivalence of the three formulations.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)

UniBE Contributor:

Rothen-Rutishauser, Barbara

ISSN:

0939-6411

ISBN:

18771729

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:03

Last Modified:

04 May 2014 23:19

Publisher DOI:

10.1016/j.ejpb.2008.07.019

PubMed ID:

18771729

Web of Science ID:

000267000800010

URI:

https://boris.unibe.ch/id/eprint/27556 (FactScience: 108821)

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