Chemokine receptor 4 (CXCR4) is part of the lipopolysaccharide "sensing apparatus"

Triantafilou, Martha; Lepper, Philipp M; Briault, Caspar David; Ahmed, Mohamed Abd Elrahman; Dmochowski, Josh M; Schumann, Christian; Triantafilou, Kathy (2008). Chemokine receptor 4 (CXCR4) is part of the lipopolysaccharide "sensing apparatus". European journal of immunology, 38(1), pp. 192-203. Weinheim: Wiley-VCH 10.1002/eji.200636821

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Recognition of bacterial lipopolysaccharide (LPS) by the innate immune system involves at least three receptor molecules: CD14, TLR4 and MD-2. Additional receptor components such as heat shock proteins, chemokine receptor 4 (CXCR4), or CD55 have been suggested to be part of this activation cluster; possibly acting as additional LPS transfer molecules. Our group has previously identified CXCR4 as a component of the "LPS-sensing apparatus". In this study we aimed to elucidate the role that CXCR4 plays in innate immune responses to LPS. Here we demonstrate that CXCR4 transfection results in responsiveness to LPS. Fluorescence correlation spectroscopy experiments further showed that LPS directly interacts with CXCR4. Our data suggest that CXCR4 is not only involved in LPS binding but is also responsible for triggering signalling, especially mitogen-activated protein kinases in response to LPS. Finally, co-clustering of CXCR4 with other LPS receptors seems to be crucial for LPS signalling, thus suggesting that CXCR4 is a functional part of the multimeric LPS "sensing apparatus".

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care

UniBE Contributor:

Lepper, Philipp

ISSN:

0014-2980

ISBN:

18081034

Publisher:

Wiley-VCH

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:04

Last Modified:

17 Mar 2015 22:18

Publisher DOI:

10.1002/eji.200636821

PubMed ID:

18081034

Web of Science ID:

000252726300025

URI:

https://boris.unibe.ch/id/eprint/27834 (FactScience: 112377)

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