A novel exposure system for the efficient and controlled deposition of aerosol particles onto cell cultures

Savi, Melanie; Kalberer, Markus; Lang, Doris; Ryser, Manuel; Fierz, Martin; Gaschen, Annina; Ricka, Jaroslav; Geiser, Marianne (2008). A novel exposure system for the efficient and controlled deposition of aerosol particles onto cell cultures. Environmental science & technology, 42(15), pp. 5667-74. Washington, D.C.: ACS Publications 10.1021/es703075q

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Epidemiologic studies have shown correlations between morbidity and particles < or = 2.5 microm generated from pollution processes and manufactured nanoparticles. Thereby nanoparticles seem to play a specific role. The interaction of particles with the lung, the main pathway of undesired particle uptake, is poorly understood. In most studies investigating these interactions in vitro, particle deposition differs greatly from the in vivo situation, causing controversial results. We present a nanoparticle deposition chamber to expose lung cells mimicking closely the particle deposition conditions in the lung. In this new deposition chamber, particles are deposited very efficiently, reproducibly, and uniformly onto the cell culture, a key aspect if cell responses are quantified in respect to the deposited particle number. In situ analyses of the lung cells, e.g., the ciliary beat frequency, indicative of the defense capability of the cells, are complemented by off-line biochemical, physiological, and morphological cell analyses.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Institute of Applied Physics
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Ryser, Manuel; Ricka, Jaroslav and Geiser, Marianne

ISSN:

0013-936X

ISBN:

18754491

Publisher:

ACS Publications

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:05

Last Modified:

18 Jan 2017 10:29

Publisher DOI:

10.1021/es703075q

PubMed ID:

18754491

Web of Science ID:

000258075100046

URI:

https://boris.unibe.ch/id/eprint/28112 (FactScience: 116948)

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