Granulocyte colony-stimulating factor increases hepatic sinusoidal perfusion during liver regeneration in mice

Sidler, Daniel; Studer, Peter; Küpper, Sebastian; Gloor, Beat; Candinas, Daniel; Haier, Jörg; Inderbitzin, Daniel (2008). Granulocyte colony-stimulating factor increases hepatic sinusoidal perfusion during liver regeneration in mice. Journal of investigative surgery, 21(2), pp. 57-64. New York, N.Y.: Taylor & Francis 10.1080/08941930701883632

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Conditioning with granulocyte colony-stimulating factor (G-CSF) promotes liver regeneration in an experimental small-for-size liver remnant mouse model. The mechanisms involved in this extraordinary G-CSF effect are unknown. The aim of this study was to investigate the influence of G-CSF on the hepatic microvasculature in the regenerating liver. The hepatic sinusoidal microvasculature and microarchitecture of the regenerating liver were evaluated by intravital microscopy in mice. Three experimental groups were compared: (1) unoperated unconditioned animals (control; n = 5), (2) animals conditioned with G-CSF 48 h after 60% partial hepatectomy (G-CSF-PH; n = 6), and (3) animals sham conditioned 48 h after 60% PH (sham-PH; n = 6). PH led to hepatocyte hypertrophy and increased hepatic sinusoidal velocity in the sham-PH and G-CSF-PH groups. Increased sinusoidal diameter and increased hepatic blood flow were observed in the G-CSF-PH group compared to the sham-PH and control groups. Furthermore, there was a strong positive correlation between spleen weight and hepatic sinusoidal diameter in the G-CSF-PH group. The increased hepatic blood flow could explain the observed benefit of G-CSF conditioning during liver regeneration. These results elucidate an unexplored aspect of pharmacological modulation of liver regeneration and motivate further experiments.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research [discontinued] 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery
UniBE Contributor:	Sidler, Daniel (A), Studer, Peter, Gloor, Beat, Candinas, Daniel, Inderbitzin, Daniel
ISSN:	0894-1939
ISBN:	18340621
Publisher:	Taylor & Francis
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:05
Last Modified:	29 Mar 2023 23:33
Publisher DOI:	10.1080/08941930701883632
PubMed ID:	18340621
Web of Science ID:	000254067900003
URI:	https://boris.unibe.ch/id/eprint/28456 (FactScience: 120851)