Granulocyte colony-stimulating factor increases hepatic sinusoidal perfusion during liver regeneration in mice

Sidler, Daniel; Studer, Peter; Küpper, Sebastian; Gloor, Beat; Candinas, Daniel; Haier, Jörg; Inderbitzin, Daniel (2008). Granulocyte colony-stimulating factor increases hepatic sinusoidal perfusion during liver regeneration in mice. Journal of investigative surgery, 21(2), pp. 57-64. New York, N.Y.: Taylor & Francis 10.1080/08941930701883632

Full text not available from this repository. (Request a copy)

Conditioning with granulocyte colony-stimulating factor (G-CSF) promotes liver regeneration in an experimental small-for-size liver remnant mouse model. The mechanisms involved in this extraordinary G-CSF effect are unknown. The aim of this study was to investigate the influence of G-CSF on the hepatic microvasculature in the regenerating liver. The hepatic sinusoidal microvasculature and microarchitecture of the regenerating liver were evaluated by intravital microscopy in mice. Three experimental groups were compared: (1) unoperated unconditioned animals (control; n = 5), (2) animals conditioned with G-CSF 48 h after 60% partial hepatectomy (G-CSF-PH; n = 6), and (3) animals sham conditioned 48 h after 60% PH (sham-PH; n = 6). PH led to hepatocyte hypertrophy and increased hepatic sinusoidal velocity in the sham-PH and G-CSF-PH groups. Increased sinusoidal diameter and increased hepatic blood flow were observed in the G-CSF-PH group compared to the sham-PH and control groups. Furthermore, there was a strong positive correlation between spleen weight and hepatic sinusoidal diameter in the G-CSF-PH group. The increased hepatic blood flow could explain the observed benefit of G-CSF conditioning during liver regeneration. These results elucidate an unexplored aspect of pharmacological modulation of liver regeneration and motivate further experiments.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research (discontinued)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery

UniBE Contributor:

Sidler, Daniel; Studer, Peter; Gloor, Beat; Candinas, Daniel and Inderbitzin, Daniel

ISSN:

0894-1939

ISBN:

18340621

Publisher:

Taylor & Francis

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:05

Last Modified:

06 Dec 2013 13:54

Publisher DOI:

10.1080/08941930701883632

PubMed ID:

18340621

Web of Science ID:

000254067900003

URI:

https://boris.unibe.ch/id/eprint/28456 (FactScience: 120851)

Actions (login required)

Edit item Edit item
Provide Feedback