Improved impurity fingerprinting of heparin by high resolution (1)H NMR spectroscopy

Bigler, Peter; Brenneisen, Rudolf (2009). Improved impurity fingerprinting of heparin by high resolution (1)H NMR spectroscopy. Journal of pharmaceutical and biomedical analysis, 49(4), pp. 1060-4. Oxford: Pergamon Press 10.1016/j.jpba.2009.01.017

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For improving the identification of potential heparin impurities such as oversulfated chondroitin sulfate (OSCS) the standard 2D (1)H-(1)H NMR NOESY was applied. Taking advantage of spin diffusion and adjusting the experimental parameters accordingly additional contaminant-specific signals of the corresponding sugar ring protons can easily be detected. These are usually hidden by the more intense heparin signals. Compared to the current 1D (1)H procedure proposed for screening commercial unfractionated heparin samples and focusing on the contaminants acetyl signals more informative and unique fingerprints may be obtained. Correspondingly measured (1)H fingerprints of a few potential impurities are given and their identification in two contaminated commercial heparin samples is demonstrated. The proposed 2D NOESY method is not intended to replace the current 1D method for detecting and quantifying heparin impurities but may be regarded as a valuable supplement for an improved and more reliable identification of these contaminants.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Phytopharmakologie, Bioanalytik & Pharmakokinetik (discontinued)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Phytopharmakologie, Bioanalytik & Pharmakokinetik (discontinued)

UniBE Contributor:

Bigler, Peter and Brenneisen, Rudolf Max

ISSN:

0731-7085

ISBN:

19211212

Publisher:

Pergamon Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:06

Last Modified:

04 May 2014 23:20

Publisher DOI:

10.1016/j.jpba.2009.01.017

PubMed ID:

19211212

Web of Science ID:

000265046200030

URI:

https://boris.unibe.ch/id/eprint/28552 (FactScience: 121288)

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