Proliferation and apoptosis in mammary epithelium during the rat oestrous cycle

Strange, R; Westerlind, K C; Ziemiecki, A; Andres, A-C (2007). Proliferation and apoptosis in mammary epithelium during the rat oestrous cycle. Acta physiologica, 190(2), pp. 137-49. Oxford: Blackwell Publishing 10.1111/j.1748-1716.2007.01704.x

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AIM: During each oestrous cycle, the mammary gland is subject to changes in ovarian hormone levels. It responds with limited proliferation, differentiation and regression. To understand the processes resulting in these changes, particularly the regulation of cell death, we examined protein levels in mammary epithelium during the oestrous cycle of the Sprague-Dawley rat. METHODS: Studies of serum hormone levels, vaginal smears, uterine weight and morphology, mammary gland morphology, proliferation and apoptotic indices, and protein levels during the stages of the Sprague-Dawley rat oestrous cycle were used to examine the response of mammary epithelium to the oestrous cycle. RESULTS: Proliferation of mammary epithelium was greater in diestrus and proestrus, while apoptosis was increased in metestrus and diestrus. Growth factor-, hormone- and anchorage-mediated cell survival signalling, indicated by activation of Stat5A, FAK and Akt 1 and expression of anti-apoptotic Bcl-2 family members, was greater in proestrus and reduced in metestrus. In contrast, the levels of pro-apoptotic Bcl-2 family members and proteins associated with apoptosis in mammary epithelium (TGFbeta3, pStat3) were increased during metestrus and diestrus. CONCLUSION: Decreases in growth factor, hormone and cell attachment survival signals corresponded with increased apoptosis during the second half of the oestrous cycle. The protein levels detected during oestrus suggest parallels to apoptosis in mammary involution.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Tiefenau Hospital [discontinued] > Forschungsgruppe Biologie und Karzinogenese der Brustdrüse [discontinued]
UniBE Contributor:	Andres, Anne Catherine
ISSN:	1748-1708
ISBN:	17433024
Publisher:	Blackwell Publishing
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:06
Last Modified:	05 Dec 2022 14:20
Publisher DOI:	10.1111/j.1748-1716.2007.01704.x
PubMed ID:	17433024
Web of Science ID:	000247565400006
URI:	https://boris.unibe.ch/id/eprint/28553 (FactScience: 121290)