Ascorbic acid for amelioration of reperfusion injury in a lung autotransplantation model in sheep

Demertzis, S; Scherer, M; Langer, F; Dwenger, A; Hausen, B; Schäfers, H J (2000). Ascorbic acid for amelioration of reperfusion injury in a lung autotransplantation model in sheep. Annals of thoracic surgery, 5(70), pp. 1684-9. New York, N.Y.: Elsevier 10.1016/S0003-4975(00)01846-4

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BACKGROUND: Reperfusion injury is the leading cause of early graft dysfunction after lung transplantation. Activation of neutrophilic granulocytes with generation of free oxygen radicals appears to play a key role in this process. The efficacy of ascorbic acid as an antioxidant in the amelioration of reperfusion injury after lung transplantation has not been studied yet. METHODS: An in situ autotransplantation model in sheep is presented. The left lung was flushed (Euro-Collins solution) and reperfused; after 2 hours of cold storage, the right hilus was then clamped (group R [reference], n = 6). Group AA animals (n = 6) were treated with 1 g/kg ascorbic acid before reperfusion. Controls (group C, n = 6) underwent hilar preparation and instrumentation only. RESULTS: In group R, arterio-alveolar oxygen difference (AaDO2) and pulmonary vascular resistance (PVR) were significantly elevated after reperfusion. Five of 6 animals developed frank alveolar edema. All biochemical parameters showed significant PMN activation. In group AA, AaDO2, PVR, work of breathing, and the level of PMN activation were significantly lower. CONCLUSIONS: The experimental model reproduces all aspects of lung reperfusion injury reliably. Ascorbic acid was able to weaken reperfusion injury in this experimental setup.

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Journal Article (Original Article)


04 Faculty of Medicine > Faculty Institutions > Teaching Staff, Faculty of Medicine

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Demertzis, Stefanos










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Date Deposited:

04 Oct 2013 15:08

Last Modified:

04 May 2014 23:21

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URI: (FactScience: 157803)

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