Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes

Luckschander, Nicole; Pfammatter, Nadia S; Sidler, Daniel; Jakob, Sabine; Burgener, Iwan A; Moore, Peter F; Zurbriggen, Andreas; Corazza, Nadia; Brunner, Thomas (2009). Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes. Veterinary research, 40(6), p. 58. Paris: Editions scientifiques Elsevier 10.1051/vetres/2009042

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Little is currently known about the lymphocyte populations in the normal and diseased canine gut. The aim of this study was thus the phenotypical and functional characterization of canine intestinal intraepithelial lymphocytes (IEL). IEL were isolated from full-thickness biopsies of 15 adult Swiss Beagle dogs (mean age 8.2 +/-2.8 years) and compared to mesenteric lymph node cells. The phenotypical characterization by multi-parameter flow cytometry revealed that canine IEL differ substantially from lymph node T cells, and consist of various unconventional lymphocyte subsets, unique to mucosal surfaces. These include gammasigma T cells, and CD4(-)CD8(-) and CD8alphaalpha(+) T cells. IEL populations in adult dogs were also compared to those isolated from neonatal Beagle dogs. Analysis revealed a high frequency of undifferentiated CD4(-)CD8(-) T cells in newborn dogs whereas mature CD4(+) and CD8(+) T cells predominate in adult dogs, indicating maturation of the intestinal immune system during development. As IEL in other species are thought to exhibit regulatory functions, we investigated the role of IEL on the activation-induced proliferation of lymph node T cells. While IEL alone did not show activation-induced proliferation, they significantly inhibited the proliferation of activated lymph node T cells in a cell number-dependent manner. These findings are the first to demonstrate that canine intestinal IEL have an immunoregulatory phenotype, which may contribute to the maintenance of intestinal immune homeostasis and may, therefore, be lost in canine chronic enteropathies.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
04 Faculty of Medicine > Service Sector > Institute of Pathology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research

UniBE Contributor:

Luckschander, Nicole; Sidler, Daniel; Burgener, Iwan; Zurbriggen, Andreas; Corazza, Nadia and Brunner, Thomas

ISSN:

0928-4249

Publisher:

Editions scientifiques Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:08

Last Modified:

21 Jan 2014 15:05

Publisher DOI:

10.1051/vetres/2009042

PubMed ID:

19631032

Web of Science ID:

000272039500007

URI:

https://boris.unibe.ch/id/eprint/29966 (FactScience: 165532)

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