In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications

Montjovent, MO; Bocelli-Tyndall, C; Scaletta, C; Scherberich, A; Mark, S; Martin, I; Applegate, LA; Pioletti, DP (2009). In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications. Tissue engineering. Part A, 15(7), pp. 1523-32. New Rochelle, N.Y.: Mary Ann Liebert 10.1089/ten.tea.2008.0222

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We describe herein some immunological properties of human fetal bone cells recently tested for bone tissue-engineering applications. Adult mesenchymal stem cells (MSCs) and osteoblasts were included in the study for comparison. Surface markers involved in bone metabolism and immune recognition were analyzed using flow cytometry before and after differentiation or treatment with cytokines. Immunomodulatory properties were studied on activated peripheral blood mononuclear cells (PBMCs). The immuno-profile of fetal bone cells was further investigated at the gene expression level. Fetal bone cells and adult MSCs were positive for Stro-1, alkaline phosphatase, CD10, CD44, CD54, and beta2-microglobulin, but human leukocyte antigen (HLA)-I and CD80 were less present than on adult osteoblasts. All cells were negative for HLA-II. Treatment with recombinant human interferon gamma increased the presence of HLA-I in adult cells much more than in fetal cells. In the presence of activated PBMCs, fetal cells had antiproliferative effects, although with patterns not always comparable with those of adult MSCs and osteoblasts. Because of the immunological profile, and with their more-differentiated phenotype than of stem cells, fetal bone cells present an interesting potential for allogeneic cell source in tissue-engineering applications.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
UniBE Contributor:	Montjovent, Marc-Oliver
ISSN:	1937-3341
ISBN:	19196143
Publisher:	Mary Ann Liebert
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:09
Last Modified:	05 Dec 2022 14:21
Publisher DOI:	10.1089/ten.tea.2008.0222
PubMed ID:	19196143
Web of Science ID:	000267843700009
URI:	https://boris.unibe.ch/id/eprint/30375 (FactScience: 193014)