Cathepsin G is differentially expressed in primary human antigen-presenting cells

Stoeckle, Christina; Sommandas, Vinod; Adamopoulou, Eleni; Belisle, Kurt; Schiekofer, Stephan; Melms, Arthur; Weber, Ekkehard; Driessen, Christoph; Boehm, Bernhard O; Tolosa, Eva; Burster, Timo (2009). Cathepsin G is differentially expressed in primary human antigen-presenting cells. Cellular immunology, 255(1-2), pp. 41-5. San Diego, Calif.: Elsevier 10.1016/j.cellimm.2008.10.001

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Cathepsins are required for the processing of antigens in order to make them suitable for loading on major histocompatibility complex (MHC) class II molecules, for subsequent presentation to CD4(+) T cells. It was shown that antigen processing in monocyte-derived dendritic cells (DC), a commonly used DC model, is different from that of primary human DC. Here, we report that the two subsets of human myeloid DC (mDC) and plasmacytoid DC (pDC) differ in their cathepsin distribution. The serine protease cathepsin G (CatG) was detected in mDC1, mDC2, pDC, cortical thymic epithelial cells (cTEC) and high levels of CatG were determined in pDC. To address the role of CatG in the processing and presentation of a Multiple Sclerosis-associated autoantigen myelin basic protein (MBP), we used a non-CatG expressing fibroblast cell line and fibroblasts, which were preloaded with purified CatG. We find that preloading fibroblasts with CatG results in a decrease of MBP84-98-specific T cell proliferation, when compared to control cells. Our data suggest a different processing signature in primary human antigen-presenting cells and CatG may be of functional importance.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

Merz, Christina

ISSN:

0008-8749

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:10

Last Modified:

04 May 2014 23:22

Publisher DOI:

10.1016/j.cellimm.2008.10.001

PubMed ID:

19036358

Web of Science ID:

000263625500006

URI:

https://boris.unibe.ch/id/eprint/30705 (FactScience: 194981)

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