Generation of an osteogenic graft from human placenta and placenta-derived mesenchymal stem cells

Mohr, Stefan; Portmann-Lanz, C Bettina; Schoeberlein, Andreina; Sager, Ruth; Surbek, Daniel V (2010). Generation of an osteogenic graft from human placenta and placenta-derived mesenchymal stem cells. Reproductive sciences, 17(11), pp. 1006-15. Thousand Oaks, Calif.: Sage 10.1177/1933719110377471

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The objective of the study was to determine the feasibility of generating a biodegradable, stem cell-loaded osteogenic composite graft from human placenta. Initially, a scaffold from human chorion membrane was produced. Human placenta mesenchymal stem cells (MSCs) derived from either first-trimester chorionic villi or term chorion membrane were differentiated osteogenically on this scaffold. Outgrowth, adherence, and osteogenic differentiation of cells were assessed by immunohistochemistry (IHC), scanning electron microscopy, protein expression, and real-time polymerase chain reaction (RT-PCR). Our results showed that a cell-free extracellular matrix scaffold can be generated from human chorion. Seeded MSCs densely adhered to that scaffold and were osteogenically differentiated. Calcium and alkaline phosphatase were detected in the cell-scaffold constructs as a proof of mineralization and findings were confirmed by IHC and RT-PCR results. This study shows for the first time that generation of an osteogenic composite graft using placental tissue is feasible. It might allow therapeutic application of autologous or allogeneic grafts in congenital skeletal defects by means of a composite graft.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin

UniBE Contributor:

Mohr, Stefan; Portmann-Lanz, Bettina; Schoeberlein, Andreina and Surbek, Daniel

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1933-7191

Publisher:

Sage

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:13

Last Modified:

28 Mar 2019 09:01

Publisher DOI:

10.1177/1933719110377471

PubMed ID:

20940246

Web of Science ID:

000282815100005

URI:

https://boris.unibe.ch/id/eprint/3148 (FactScience: 206620)

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