Spichiger, Miriam; Mühlbauer, Roman C; Brenneisen, Rudolf (2004). Determination of menthol in plasma and urine of rats and humans by headspace solid phase microextraction and gas chromatography--mass spectrometry. Journal of chromatography. B - analytical technologies in the biomedical and life sciences, 799(1), pp. 111-7. New York, N.Y.: Elsevier 10.1016/j.jchromb.2003.10.022
Full text not available from this repository.A method for the determination of menthol and menthol glucuronide (M-G) after enzymatic hydrolysis in plasma and urine of rats and humans was developed using headspace solid phase microextraction and gas chromatography-mass spectrometry in the selected ion monitoring mode (HS-SPME/GC-MS). The assay linearity for plasma ranged from 5 to 1000 ng/ml. The limit of quantification (LOQ) in plasma was 5 ng/ml. The intra- and inter-day precision for menthol and M-G were < or = 18.1% R.S.D. at the LOQ and < or = 4.0% at higher concentrations. Menthol and M-G were determined in rat and human plasma and urine after administration of menthol.