Determination of ajulemic acid and its glucuronide in human plasma by gas chromatography-mass spectrometry

Batista, Catarina; Berisha, Myftar; Karst, Matthias; Salim, Kahlid; Schneider, Udo; Brenneisen, Rudolf (2005). Determination of ajulemic acid and its glucuronide in human plasma by gas chromatography-mass spectrometry. Journal of chromatography. B - analytical technologies in the biomedical and life sciences, 820(1), pp. 77-82. New York, N.Y.: Elsevier 10.1016/j.jchromb.2005.03.013

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A method using gas chromatography-mass spectrometry (GC-MS) and solid-phase extraction (SPE) was developed for the determination of ajulemic acid (AJA), a non-psychoactive synthetic cannabinoid with interesting therapeutic potential, in human plasma. When using two calibration graphs, the assay linearity ranged from 10 to 750 ng/ml, and 750 to 3000 ng/ml AJA. The intra- and inter-day precision (R.S.D., %), assessed across the linear ranges of the assay, was between 1.5 and 7.0, and 3.6 and 7.9, respectively. The limit of quantitation (LOQ) was 10 ng/ml. The amount of AJA glucuronide was determined by calculating the difference in the AJA concentration before ("free AJA") and after enzymatic hydrolysis ("total AJA"). The present method was used within a clinical study on 21 patients suffering from neuropathic pain with hyperalgesia and allodynia. For example, plasma levels of 599.4+/-37.2 ng/ml (mean+/-R.S.D., n=9) AJA were obtained for samples taken 2 h after the administration of an oral dose of 20 mg AJA. The mean AJA glucuronide concentration at 2h was 63.8+/-127.9 ng/ml.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Phytopharmakologie, Bioanalytik & Pharmakokinetik [discontinued]
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Phytopharmakologie, Bioanalytik & Pharmakokinetik [discontinued]

UniBE Contributor:

Brenneisen, Rudolf Max

ISSN:

1570-0232

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:12

Last Modified:

06 Dec 2013 13:58

Publisher DOI:

10.1016/j.jchromb.2005.03.013

PubMed ID:

15866495

Web of Science ID:

000229167000010

URI:

https://boris.unibe.ch/id/eprint/31659 (FactScience: 196307)

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