Leukemia inhibitory factor favours neurogenic differentiation of long-term propagated human midbrain precursor cells

Andersen, Rikke K; Widmer, Hans R; Zimmer, Jens; Wahlberg, Lars U; Meyer, Morten (2009). Leukemia inhibitory factor favours neurogenic differentiation of long-term propagated human midbrain precursor cells. Neuroscience letters, 464(3), pp. 203-8. Amsterdam: Elsevier 10.1016/j.neulet.2009.08.050

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There is a lot of excitement about the potential use of multipotent neural stem cells for the treatment of neurodegenerative diseases. However, the strategy is compromised by the general loss of multipotency and ability to generate neurons after long-term in vitro propagation. In the present study, human embryonic (5 weeks post-conception) ventral mesencephalic (VM) precursor cells were propagated as neural tissue-spheres (NTS) in epidermal growth factor (EGF; 20 ng/ml) and fibroblast growth factor 2 (FGF2; 20 ng/ml). After more than 325 days, the NTS were transferred to media containing either EGF+FGF2, EGF+FGF2+heparin or leukemia inhibitory factor (LIF; 10 ng/ml)+FGF2+heparin. Cultures were subsequently propagated for more than 180 days with NTS analyzed at various time-points. Our data show for the first time that human VM neural precursor cells can be long-term propagated as NTS in the presence of EGF and FGF2. A positive effect of heparin was found only after 150 days of treatment. After switching into different media, only NTS exposed to LIF contained numerous cells positive for markers of newly formed neurons. Besides of demonstrating the ability of human VM NTS to be long-term propagated, our study also suggests that LIF favours neurogenic differentiation of human VM precursor cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery

UniBE Contributor:

Widmer, Hans Rudolf

ISSN:

0304-3940

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:13

Last Modified:

04 May 2014 23:23

Publisher DOI:

10.1016/j.neulet.2009.08.050

PubMed ID:

19703518

Web of Science ID:

000270659500012

URI:

https://boris.unibe.ch/id/eprint/32026 (FactScience: 196854)

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