Identification of small Sca-1(+), Lin(-), CD45(-) multipotential cells in the neonatal murine retina

Liu, Yongqing; Gao, Ling; Zuba-Surma, Ewa K; Peng, Xiaoyan; Kucia, Magdalena; Ratajczak, Mariusz Z; Wang, Wei; Enzmann, Volker; Enzman, Volker; Kaplan, Henry J; Dean, Douglas C (2009). Identification of small Sca-1(+), Lin(-), CD45(-) multipotential cells in the neonatal murine retina. Experimental hematology, 37(9), 1096-107, 1107.e1. New York, N.Y.: Elsevier 10.1016/j.exphem.2009.05.014

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OBJECTIVE: Bone marrow contains a subset of stem cells that give rise to nonhematopoietic lineages. These nonhematopoietic stem cells appear heterogeneous and contain cells committed to mesenchymal and endothelial lineages, as well as more primitive multipotential cells resembling progenitors of germ cells and very small embryonic/epiblast-like stem cells (VSELs). Nonhematopoietic stem cells can be mobilized from the bone marrow in response to tissue injury, and cells with similar properties have been found in cord blood and normal adult organs. However, the relationship between bone marrow cells and these adult organ stem cells is still unclear. The differentiation potential of some adult stem cells is organ-restricted, but other populations appear to retain multipotential capacity. MATERIALS AND METHODS: A population of small Sca-1(+), lineage-negative (Lin(-)), CD45(-) cells resembling VSELs were isolated from neonatal mouse retina by cell sorting. Differentiation of the cells in culture was achieved by exposure to embryonic stem cell differentiation protocols. RESULTS: VSEL-like cells comprise 1.5% of the neonatal mouse retina. They remain quiescent during retinal differentiation, and thus they do not contribute to normal retinal development. However, they display eye cell differentiation potential in culture and they are also multipotential and can give rise to cells representative of all three embryonic layers. CONCLUSIONS: The neonatal retina is an abundant postnatal source of multipotential VSEL-like cells that can differentiate in culture into a variety of lineages.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology
UniBE Contributor:	Enzmann, Volker
ISSN:	0301-472X
Publisher:	Elsevier
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:13
Last Modified:	05 Dec 2022 14:22
Publisher DOI:	10.1016/j.exphem.2009.05.014
PubMed ID:	19539690
Web of Science ID:	000269396200010
URI:	https://boris.unibe.ch/id/eprint/32244 (FactScience: 197257)