Novel autoantigens immunogenic in COPD patients

Leidinger, P; Keller, A; Heisel, S; Ludwig, N; Rheinheimer, S; Klein, V; Andres, C; Hamacher, J; Huwer, H; Stephan, B; Stehle, I; Lenhof, HP; Meese, E (2009). Novel autoantigens immunogenic in COPD patients. Respiratory research, 12(10), p. 20. London: BioMed Central 10.1186/1465-9921-10-20

[img]
Preview
Text
2.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (361kB) | Preview

Background Chronic obstructive pulmonary disease (COPD) is a respiratory inflammatory condition with autoimmune features including IgG autoantibodies. In this study we analyze the complexity of the autoantibody response and reveal the nature of the antigens that are recognized by autoantibodies in COPD patients. Methods An array of 1827 gridded immunogenic peptide clones was established and screened with 17 sera of COPD patients and 60 healthy controls. Protein arrays were evaluated both by visual inspection and a recently developed computer aided image analysis technique. By this computer aided image analysis technique we computed the intensity values for each peptide clone and each serum and calculated the area under the receiver operator characteristics curve (AUC) for each clone and the separation COPD sera versus control sera. Results By visual evaluation we detected 381 peptide clones that reacted with autoantibodies of COPD patients including 17 clones that reacted with more than 60% of the COPD sera and seven clones that reacted with more than 90% of the COPD sera. The comparison of COPD sera and controls by the automated image analysis system identified 212 peptide clones with informative AUC values. By in silico sequence analysis we found an enrichment of sequence motives previously associated with immunogenicity. Conclusion The identification of a rather complex humoral immune response in COPD patients supports the idea of COPD as a disease with strong autoimmune features. The identification of novel immunogenic antigens is a first step towards a better understanding of the autoimmune component of COPD.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Hamacher, Jürg

ISSN:

1465-9921

Publisher:

BioMed Central

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:13

Last Modified:

30 Dec 2014 07:36

Publisher DOI:

10.1186/1465-9921-10-20

Web of Science ID:

000265394100001

BORIS DOI:

10.7892/boris.32290

URI:

https://boris.unibe.ch/id/eprint/32290 (FactScience: 197342)

Actions (login required)

Edit item Edit item
Provide Feedback