Simultaneous quantitative detection of relevant biomarkers in breast cancer by quantitative real-time PCR

Labuhn, M; Vuaroqueaux, V; Fina, F; Schaller, A; Nanni-Metellus, I; Kung, W; Eppenberger-Castori, S; Martin, P M; Eppenberger, U (2006). Simultaneous quantitative detection of relevant biomarkers in breast cancer by quantitative real-time PCR. International journal of biological markers, 21(1), pp. 30-9. Milano: Wichtig Editore

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The assessment of ERa, PgR and HER2 status is routinely performed today to determine the endocrine responsiveness of breast cancer samples. Such determination is usually accomplished by means of immunohistochemistry and in case of HER2 amplification by means of fluorescent in situ hybridization (FISH). The analysis of these markers can be improved by simultaneous measurements using quantitative real-time PCR (Qrt-PCR). In this study we compared Qrt-PCR results for the assessment of mRNA levels of ERa, PgR, and the members of the human epidermal growth factor receptor family, HER1, HER2, HER3 and HER4. The results were obtained in two independent laboratories using two different methods, SYBR Green I and TaqMan probes, and different primers. By linear regression we demonstrated a good concordance for all six markers. The quantitative mRNA expression levels of ERa, PgR and HER2 also strongly correlated with the respective quantitative protein expression levels prospectively detected by EIA in both laboratories. In addition, HER2 mRNA expression levels correlated well with gene amplification detected by FISH in the same biopsies. Our results indicate that both Qrt-PCR methods were robust and sensitive tools for routine diagnostics and consistent with standard methodologies. The developed simultaneous assessment of several biomarkers is fast and labor effective and allows optimization of the clinical decision-making process in breast cancer tissue and/or core biopsies.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine

UniBE Contributor:

Schaller, André

ISSN:

0393-6155

Publisher:

Wichtig Editore

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:13

Last Modified:

07 Nov 2014 10:24

PubMed ID:

16711511

Web of Science ID:

000236476500005

URI:

https://boris.unibe.ch/id/eprint/32310 (FactScience: 197374)

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