Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export

Ruepp, Marc-David; Aringhieri, Chiara; Vivarelli, Silvia; Cardinale, Stefano; Paro, Simona; Schümperli, Daniel; Barabino, Silvia M.L. (2009). Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export. Molecular biology of the cell, 20(24), pp. 5211-23. Bethesda, Md.: American Society for Cell Biology 10.1091/mbc.E09-05-0389

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Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between mRNA 3' end formation and export, it is not known how these two processes are mechanistically connected. Here, we show that a subunit of the mammalian pre-mRNA 3' end processing complex, CF I(m)68, stimulates mRNA export. CF I(m)68 shuttles between the nucleus and the cytoplasm in a transcription-dependent manner and interacts with the mRNA export receptor NXF1/TAP. Consistent with the idea that CF I(m)68 may act as a novel adaptor for NXF1/TAP, we show that CF I(m)68 promotes the export of a reporter mRNA as well as of endogenous mRNAs, whereas silencing by RNAi results in the accumulation of mRNAs in the nucleus. Moreover, CF I(m)68 associates with 80S ribosomes but not polysomes, suggesting that it is part of the mRNP that is remodeled in the cytoplasm during the initial stages of translation. These results reveal a novel function for the pre-mRNA 3' end processing factor CF I(m)68 in mRNA export.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Ruepp, Marc-David and Schümperli, Daniel

ISSN:

1059-1524

Publisher:

American Society for Cell Biology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:21

Last Modified:

17 Mar 2015 22:46

Publisher DOI:

10.1091/mbc.E09-05-0389

Web of Science ID:

000272709800015

URI:

https://boris.unibe.ch/id/eprint/36693 (FactScience: 205864)

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