Hartsuiker, Edgar; Mizuno, Kenichi; Molnar, Monika; Kohli, Jürg; Ohta, Kunihiro; Carr, Antony M. (2009). Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions. Molecular and cellular biology, 29(7), pp. 1671-81. Washington, D.C.: American Society for Microbiology 10.1128/MCB.01182-08
Full text not available from this repository.The evolutionarily conserved Mre11/Rad50/Nbs1 (MRN) complex is involved in various aspects of meiosis. Whereas available evidence suggests that the Mre11 nuclease activity might be responsible for Spo11 removal in Saccharomyces cerevisiae, this has not been confirmed experimentally. This study demonstrates for the first time that Mre11 (Schizosaccharomyces pombe Rad32(Mre11)) nuclease activity is required for the removal of Rec12(Spo11). Furthermore, we show that the CtIP homologue Ctp1 is required for Rec12(Spo11) removal, confirming functional conservation between Ctp1(CtIP) and the more distantly related Sae2 protein from Saccharomyces cerevisiae. Finally, we show that the MRN complex is required for meiotic recombination, chromatin remodeling at the ade6-M26 recombination hot spot, and formation of linear elements (which are the equivalent of the synaptonemal complex found in other eukaryotes) but that all of these functions are proficient in a rad50S mutant, which is deficient for Rec12(Spo11) removal. These observations suggest that the conserved role of the MRN complex in these meiotic functions is independent of Rec12(Spo11) removal.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Kohli, Jürg |
ISSN: |
0270-7306 |
Publisher: |
American Society for Microbiology |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:21 |
Last Modified: |
05 Dec 2022 14:25 |
Publisher DOI: |
10.1128/MCB.01182-08 |
Web of Science ID: |
000264080900001 |
Additional Information: |
C20600/A6620/Cancer Research UK/United Kingdom;Medical Research Council/United Kingdom;Journal Article;Research Support, Non-U.S. Gov't;United States;peer-reviewed |
URI: |
https://boris.unibe.ch/id/eprint/36694 (FactScience: 205876) |
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