Morphological and immunocytochemical analysis of Escherichia coli-specific surface antigens in wildtype strains and in recombinant Vibrio cholerae

Lüdi, Stefan; Frey, Joachim; Favre, Didier; Viret, Jean-François; Ziethlow, Verena; Kühni, Kathrin; Stoffel, Michael Hubert (2008). Morphological and immunocytochemical analysis of Escherichia coli-specific surface antigens in wildtype strains and in recombinant Vibrio cholerae. Antonie van Leeuwenhoek - international journal of general and molecular microbiology, 93(1-2), pp. 185-192. Dordrecht: Springer Netherlands 10.1007/s10482-007-9192-0

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Adhesion is the first step in the pathogenesis of enterotoxigenic Escherichia coli infections. The genes encoding the most prevalent adhesion factors CFA/I, CS3 and CS6 were cloned into Vibrio cholerae strain CVD 103-HgR and expression of fimbriae was investigated in wildtype and recombinant strains by transmission electron microscopy in conjunction with immunolabelling and negative staining. Negative staining was effective in revealing CFA/I and CS3, but not CS6. Although morphology of fimbriae differed between wildtype and recombinant strains, corresponding surface antigens were recognized by specific antibodies. The present study provides evidence that ETEC-specific fimbriae can adequately be expressed in an attenuated V. cholerae vaccine strain and that immunoelectron microscopy is a critical tool to validate the surface expression of antigens in view of their possible suitability for recombinant vaccines.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Frey, Joachim; Kühni, Kathrin and Stoffel, Michael Hubert

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0003-6072

Publisher:

Springer Netherlands

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:24

Last Modified:

26 Jun 2018 13:16

Publisher DOI:

10.1007/s10482-007-9192-0

Web of Science ID:

000251648200017

BORIS DOI:

10.7892/boris.37770

URI:

https://boris.unibe.ch/id/eprint/37770 (FactScience: 210656)

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