Ziethlow, Verena; Favre, D.; Viret, J.F.; Frey, Joachim; Stoffel, Michael Hubert (2008). Assessment by electron-microscopy of recombinant Vibrio cholerae and Salmonella vaccine strains expressing enterotoxigenic Escherichia coli-specific surface antigens. Clinical microbiology and infection, 14(3), pp. 282-286. Oxford: Blackwell Publishing 10.1111/j.1469-0691.2007.01908.x
Full text not available from this repository. (Request a copy)Diarrhoea caused by enterotoxigenic Escherichia coli (ETEC) requires adhesion of microorganisms to enterocytes. Hence, a promising approach to immunoprophylaxis is to elicit antibodies against colonisation factor antigens (CFAs). Genes encoding the most prevalent ETEC-specific surface antigens were cloned into Vibrio cholerae and Salmonella vaccine strains. Expression of surface antigens was assessed by electron-microscopy. Whereas negative staining was effective in revealing CFA/I and CS3, but not CS6, immunolabelling allowed identification of all surface antigens examined. The V. cholerae vaccine strain CVD103 did not express ETEC-specific colonisation factors, whereas CVD103-HgR expressed CS3 only. However, expression of both CFA/I and CS3 was demonstrated in Salmonella Ty21a.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Ziethlow, Verena, Frey, Joachim, Stoffel, Michael Hubert |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 630 Agriculture |
ISSN: |
1198-743X |
Publisher: |
Blackwell Publishing |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:24 |
Last Modified: |
05 Dec 2022 14:26 |
Publisher DOI: |
10.1111/j.1469-0691.2007.01908.x |
Web of Science ID: |
000252807300014 |
URI: |
https://boris.unibe.ch/id/eprint/37773 (FactScience: 210659) |
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