Expression and function of 5-HT7 receptors in smooth muscle preparations from equine duodenum, ileum, and pelvic flexure

Prause, Andrea S.; Stoffel, Michael Hubert; Portier, Christopher J.; Mevissen, Meike (2009). Expression and function of 5-HT7 receptors in smooth muscle preparations from equine duodenum, ileum, and pelvic flexure. Research in veterinary science, 87(2), pp. 292-299. London: Association for Veterinary Teaching and Research Work 10.1016/j.rvsc.2009.03.009

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In horses, gastrointestinal (GI) disorders occur frequently and cause a considerable demand for efficient medication. 5-Hydroxytryptamine receptors (5-HT) have been reported to be involved in GI tract motility and thus, are potential targets for treating functional bowel disorders. Our studies extend current knowledge on the 5-HT(7) receptor in equine duodenum, ileum and pelvic flexure by studying its expression throughout the intestine and its role in modulating contractility in vitro by immunofluorescence and organ bath experiments, respectively. 5-HT(7) immunoreactivity was demonstrated in both smooth muscle layers, particularly in the circular one, and within the myenteric plexus. Interstitial cells of Cajal (ICC), identified by c-Kit labeling, show a staining pattern similar to that of 5-HT(7) immunoreactivity. The selective 5-HT(7) receptor antagonist SB-269970 increased the amplitude of contractions in spontaneous contracting specimens of the ileum and in electrical field-stimulated specimens of the pelvic flexure concentration-dependently. Our in vitro experiments suggest an involvement of the 5-HT(7) receptor subtype in contractility of equine intestine. While the 5-HT(7) receptor has been established to be constitutively active and inhibits smooth muscle contractility, our experiments demonstrate an increase in contractility by the 5-HT(7) receptor ligand SB-269970, suggesting it exerting inverse agonist properties.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Pharmacology and Toxicology
UniBE Contributor:	Stoffel, Michael Hubert, Mevissen, Meike
Subjects:	500 Science > 570 Life sciences; biology 600 Technology > 630 Agriculture
ISSN:	0034-5288
Publisher:	Association for Veterinary Teaching and Research Work
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:24
Last Modified:	05 Dec 2022 14:26
Publisher DOI:	10.1016/j.rvsc.2009.03.009
Web of Science ID:	000268900700029
URI:	https://boris.unibe.ch/id/eprint/37776 (FactScience: 210662)