The Mycoplasma conjunctivae genome sequencing, annotation and analysis

Calderon-Copete, S.P.; Wigger, G.; Wunderlin, C.; Schmidheini, T.; Frey, J.; Quail, M.A.; Falquet, L. (2009). The Mycoplasma conjunctivae genome sequencing, annotation and analysis. BMC bioinformatics, 10(Suppl 6), S7. London: BioMed Central 10.1186/1471-2105-10-S6-S7

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BACKGROUND: The mollicute Mycoplasma conjunctivae is the etiological agent leading to infectious keratoconjunctivitis (IKC) in domestic sheep and wild caprinae. Although this pathogen is relatively benign for domestic animals treated by antibiotics, it can lead wild animals to blindness and death. This is a major cause of death in the protected species in the Alps (e.g., Capra ibex, Rupicapra rupicapra). METHODS: The genome was sequenced using a combined technique of GS-FLX (454) and Sanger sequencing, and annotated by an automatic pipeline that we designed using several tools interconnected via PERL scripts. The resulting annotations are stored in a MySQL database. RESULTS: The annotated sequence is deposited in the EMBL database (FM864216) and uploaded into the mollicutes database MolliGen http://cbi.labri.fr/outils/molligen/ allowing for comparative genomics. CONCLUSION: We show that our automatic pipeline allows for annotating a complete mycoplasma genome and present several examples of analysis in search for biological targets (e.g., pathogenic proteins).

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Frey, Joachim

ISSN:

1471-2105

Publisher:

BioMed Central

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:24

Last Modified:

26 Jun 2016 01:45

Publisher DOI:

10.1186/1471-2105-10-S6-S7

Web of Science ID:

000267522200007

BORIS DOI:

10.7892/boris.38221

URI:

https://boris.unibe.ch/id/eprint/38221 (FactScience: 220739)

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