Immune response of horses to vaccination with the recombinant Hc domain of botulinum neurotoxin types C and D

Stahl, C.; Unger, Lucia; Mazuet, C.; Popoff, M.; Straub, R.; Frey, J. (2009). Immune response of horses to vaccination with the recombinant Hc domain of botulinum neurotoxin types C and D. Vaccine, 27(41), pp. 5661-6. Amsterdam: Elsevier 10.1016/j.vaccine.2009.07.021

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Botulinum neurotoxins, predominantly serotypes C and D, cause equine botulism through forage poisoning. The C-terminal part of the heavy chain of botulinum neurotoxin types C and D (HcBoNT/C and D) was expressed in Escherichia coli and evaluated as a recombinant mono- and bivalent vaccine in twelve horses in comparison to a commercially available toxoid vaccine. A three-dose subcutaneous immunization of adult horses elicited robust serum antibody response in an ELISA using the immunogen as a capture antigen. Immune sera showed dose-dependent high potency in neutralizing specifically the active BoNT/C and D in the mouse protection assay. The aluminium hydroxide based mono- and bivalent recombinant HcBoNT/C and D vaccines were characterized by good compatibility and the ability to elicit protective antibody titers similar or superior to the commercially available toxoid vaccine.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Stahl, Maria Christina; Unger, Lucia; Straub, Reto and Frey, Joachim

ISSN:

0264-410X

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:24

Last Modified:

11 Sep 2014 21:23

Publisher DOI:

10.1016/j.vaccine.2009.07.021

Web of Science ID:

000270070400017

URI:

https://boris.unibe.ch/id/eprint/38229 (FactScience: 220747)

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