Biochemical typing of pathological prion protein in aging cattle with BSE

Tester, Seraina; Juillerat, Valérie; Doherr, Marcus; Haase, Bianca; Polak, M.; Ehrensperger, F.; Leeb, Tosso; Zurbriggen, Andreas; Seuberlich, Torsten (2009). Biochemical typing of pathological prion protein in aging cattle with BSE. Virology journal, 6(1), p. 64. London: BioMed Central 10.1186/1743-422X-6-64

[img]
Preview
Text
1.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (955kB) | Preview

BACKGROUND: The broad enforcement of active surveillance for bovine spongiform encephalopathy (BSE) in 2000 led to the discovery of previously unnoticed, atypical BSE phenotypes in aged cattle that differed from classical BSE (C-type) in biochemical properties of the pathological prion protein. Depending on the molecular mass and the degree of glycosylation of its proteinase K resistant core fragment (PrPres), mainly determined in samples derived from the medulla oblongata, these atypical cases are currently classified into low (L)-type or high (H)-type BSE. In the present study we address the question to what extent such atypical BSE cases are part of the BSE epidemic in Switzerland. RESULTS: To this end we analyzed the biochemical PrPres type by Western blot in a total of 33 BSE cases in cattle with a minimum age of eight years, targeting up to ten different brain regions. Our work confirmed H-type BSE in a zebu but classified all other cases as C-type BSE; indicating a very low incidence of H- and L-type BSE in Switzerland. It was documented for the first time that the biochemical PrPres type was consistent across different brain regions of aging animals with C-type and H-type BSE, i.e. independent of the neuroanatomical structure investigated. CONCLUSION: Taken together this study provides further characteristics of the BSE epidemic in Switzerland and generates new baseline data for the definition of C- and H-type BSE phenotypes, thereby underpinning the notion that they indeed represent distinct prion disease entities.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute

UniBE Contributor:

Tester, Seraina; Juillerat, Valérie; Doherr, Marcus; Haase, Bianca; Leeb, Tosso; Zurbriggen, Andreas and Seuberlich, Torsten

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

1743-422X

Publisher:

BioMed Central

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:25

Last Modified:

26 Jun 2016 01:45

Publisher DOI:

10.1186/1743-422X-6-64

Web of Science ID:

000267471800001

BORIS DOI:

10.7892/boris.38252

URI:

https://boris.unibe.ch/id/eprint/38252 (FactScience: 220771)

Actions (login required)

Edit item Edit item
Provide Feedback